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The following electronic communication was sent on Sunday, 31 January 2021 via hubNashville to the Metro Nashville Department of Health:

Please note that the request was modified from the request made online via WhatDoTheyKnow: Make and browse Freedom of Information (FOI) requests entitled: Public Health England Documents held showing SARS–COV2 has been isolated and Causes COVID–19

Greetings Sheila Hobson, I want to thank you for your prompt response to my question. I'm sorry for my delayed reply. I just want to confirm that you understood my previous e–mail so I am enclosing it below:

Has the Metro Nashville Department of Health properly isolated the SARS–COV–2 virus directly from a sample taken from a diseased patient where the patient sample was not first combined with any other source of genetic material (i.e. monkey kidney cells aka vero cells, liver cancer cells, etc.)?

Please note that I am using “isolation” in the everyday sense of the word: the act of separating a thing(s) from everything else. I am not referring to the “isolation of SARS–COV–2” as it relates to:

–the culturing of something,

–the performance of an amplification test (i.e. a [RT–]PCR test), or

–the sequencing of something.

Thank you in advance.

With your response, are you saying that the Metro Nashville Health Department has properly isolated the SARS–COV–2 virus which was also present in “patient zero” in Wuhan, China? How was this confirmation achieved? Does the Metro Nashville Health Department have a sample of the isolated SARS–COV–2 virus from “patient zero” in Wuhan, China? If not, then what was used for this confirmation? Essentially, I am trying to determine if Postulate 2 of Robert Koch's Postulates has been satisfied. Below I am enclosing his 4 postulates:
  1. The germ must be found in all cases of the disease.
  2. The germ must be isolated from the host and grown in pure culture.
  3. The purified germ must cause the disease again in another host.
  4. The germ must then be isolated from the newly infected host.
I want to thank you in advance. Irucka Embry

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